MDQ1
 |   |  |
 | Step-wise protocol for using (Cat. No. MDQ1) |
Image
Useful Links & Tools
- MSDS
- Certificate of Analysis
- MDQ1 - Bulletin (94 KB)
- Bulk Quote
- Similar Products
MDQ1
Sigma
Imprint® Methylated DNA Quantification Kit
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Features and Benefits | • ELISA based format • Procedure completes in 4 easy to follow steps • All reagents supplied including control methylated DNA • Detection limit is 5 ng of fully methylated DNA • Input DNA may be as low as 10-200 ng • Procedure completes in 3.5 hours • 96 well format allows option of studying single samples or high-throughput studies • May be used with cells, tissues, plasma and other body fluids |
| General description | Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured. |
| Legal Information | Imprint is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Safety
| Hazard Codes | Xn |
| Risk Statements | 20/21/22-33 |
| Safety Statements | 36/37 |
Components
| Kit component only | 10x Wash Buffer |
| DNA Binding Solution | |
| Methylated Control DNA (50 ng/μL) | |
| Block Solution | |
| Capture Antibody | |
| Detection Antibody | |
| Developing Solution | |
| Stop Solution | |
| 8 well Assay Strips |
Related Products
| Related product | H7789, Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit |
| SH3011, MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock | |
| H2663, Monoclonal Anti-Histone Deacetylase 2 (HDAC2) antibody produced in mouse | |
| H0913, Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse | |
| H7162, Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit | |
| H9286, Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit | |
| MOD50, Imprint® DNA Modification Kit | |
| A5463, Anti-Acetylated Proteins antibody produced in rabbit | |
| H9161, Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit | |
| R4279, Anti-RbAp46, C-terminal antibody produced in rabbit | |
| CHP1, Imprint® Chromatin Immunoprecipitation Kit |
References
| reference | Thaler, R., et al., Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet. Br. J. Nutr. 7, 1-7, (2008) |
| Suzuki, M., et al., Zebularine-induced reduction in VEGF secretion by HIF-1a degradation in oral squamous cell carcinoma. Molec. Med. Rep. 1, 465-471, (2008) | |
| Novikova, S.I., et al., Maternal cocaine administration in mice alters DNA methylation and gene expression in hippocampal neurons of neonatal and prepubertal offspring. PLoS ONE 3(4), e1919, (2008) | |
| Liu, C., et al., Plasma S-adenosylhomocysteine is a better biomarker of atherosclerosis than homocysteine in apolipoprotein E-deficient mice fed high dietary methionine. J. Nutr. 138(2), 311-315, (2008) |
